The effect of starvation on protein synthesis in
Caenorhabditis elegans was studied in this experiment. Worms were kept well fed
(C, control) or starved (S) for 6 hours. Ribosomal fractions were isolated from
the two groups and centrifuged in separate tubes through 1560% sucrose density
gradients. Fractions were collected from the top of the sucrose gradient. UV
absorption at 260 nm (OD260) was measured (A), and RNA isolated from
each fraction was analyzed by agarose gel electrophoresis (B).
What complexes are detected by UV absorption in fractions
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