State THREE Differences Between Genomic DNA Library And cDNA Library.

State THREE differences between genomic DNA library and cDNA library. (ii) Explain the disadvantage of genomic DNA library as compared to cDNA library in searching for gene sequences. (b) A polymerase chain reaction (PCR) was carried out to amplify a 500 base-pair gene fragment from a bacterial genome with the optimal thermal cycle profile of 94°C (30 seconds), 50°C (30 seconds), and 72°C (45 seconds) for 30 cycles. (i) Describe the processes occurred at 94°C, 50°C, and 72°C, respectively. (ii) Assuming that the PCR occurs at 100% efficiency, calculate the total number of amplicon that will be generated if 4 copies of DNA template were used in the reaction. Show your working. (iii) Name a suitable polymerase for the PCR reaction if you wish to clone the amplified 500 base-pair DNA gene fragment into a TA cloning vector. Explain your answer. (C) List TWO differences between Southern and western blotting.
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