Promelittin (PM) is a small secretory protein, a predominant component of queen bee venom. Its…

Promelittin (PM) is a small secretory protein, a predominant
component of queen bee venom. Its synthesis and posttranslational metabolism
was studied in an in vitro translation system containing total bee venom gland
mRNA, [3 H]proline, [35S]methionine, and other molecules required for protein
synthesis. Supplements were added to this system as follows: • Sample 1: Rough
ER vesicles (rough microsomes, RM)

• Sample 2: No additional components

• Sample 3: Cycloheximide (a protein synthesis inhibitor)
was added at the end of translation, followed by the addition of rough ER
vesicles (RM)
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Promelittin (PM) is a small secretory protein, a predominant
component of queen bee venom. Its synthesis and posttranslational metabolism
was studied in an in vitro translation system containing total bee venom gland
mRNA, [3 H]proline, [35S]methionine, and other molecules required for protein
synthesis. Supplements were added to this system as follows: • Sample 1: Rough
ER vesicles (rough microsomes, RM)

• Sample 2: No additional components

• Sample 3: Cycloheximide (a protein synthesis inhibitor)
was added at the end of translation, followed by the addition of rough ER
vesicles (RM) and another 30-minute incubation.

• Sample 4: Rough ER vesicles (RM-E) treated with
N-ethylmaleimide (an inhibitor of SRP receptor) were added at the beginning of
translation.

• Sample 5: Same as sample 4, except this sample was
digested with proteinase K after translation

The samples were subjected to SDS-polyacrylamide gel
electrophoresis followed by autoradiography

. Explain how the bands pPM and PM were resolved and suggest
a possible relationship between them.

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