How is intracellular Ca2+ level controlled by extracellular signals? Can you design two simple experiments to test if the secondary messenger, CAMP is involved in this Ca2+ release fro ER? Can design a simple experiment to examine if the increased intracellular Ca2+ come from extracellular fluid or intracellular Caztreservoirs following ligand stimulation? Phorbol esters, activators of protein kinase C can promote tumorigenesis, what happen to the cells with over-activation of Gq? How arc PLCB and PLCy activated? Why is PIP2 a focal point for two different signalling.
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