Experiment Hemoglobin S and hemoglobin C are abnormal forms of normal hemoglobin (hemoglobin A)….

Experiment

Hemoglobin S and hemoglobin C are abnormal forms of normal
hemoglobin (hemoglobin A). Both are caused by a point mutation in the
_-globin gene. Hemoglobin S is the main form of hemoglobin in the blood of
sickle cell anemia patients; hemoglobin C causes a rather mild form of
hemoglobinopathy. Red blood cell extracts from six individuals with different
hemoglobin types were subjected to paper electrophoresis. The filter was then
stained with a protein dye. Samples 4 and 5 are from the same patient, before
(4) and after (5) a blood transfusion. (The origin of each sample was at
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Experiment

Hemoglobin S and hemoglobin C are abnormal forms of normal
hemoglobin (hemoglobin A). Both are caused by a point mutation in the
_-globin gene. Hemoglobin S is the main form of hemoglobin in the blood of
sickle cell anemia patients; hemoglobin C causes a rather mild form of
hemoglobinopathy. Red blood cell extracts from six individuals with different
hemoglobin types were subjected to paper electrophoresis. The filter was then
stained with a protein dye. Samples 4 and 5 are from the same patient, before
(4) and after (5) a blood transfusion. (The origin of each sample was at the
top of the membrane, as the arrow indicates; – and + indicate the positions and
charges of the electrodes during electrophoresis.)

 

Each patient has two _-globin genes, one on each
homologous chromosome. What genes are present in each patient? Which patient
would you expect to be most ill and which patient most healthy?

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