design the forward and reverse primer, write in the 5′ to 3’direction and design it to be 6 nucleotides long . calculate the Tmof each primer you design and the annealing temperature you woulduse in PCR. add and EcoR1 cut site to the forward primer, and BamH1cut site to the reverse primer. Be sure to underline / label theenzyme recognition sites. 5-ATGGGAGGTCCTGCGTTAGTCTTTAA-3
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