Binary fission and budding are the exact same thing. (a) true (b) false 46. Which of the following is true of the lag phase of microbial growth? (a) organisms do not increase in size (b) organisms are metabolically active (c) organisms are dying from depletion of nutrients (d) all of the above 47. If the generation time of a given bacteria is 60 minutes and the original culture had 300 cells/mL, what would be the concentration of bacterial cells after 2 hours? (a) 300 cells/mL (b) 600 cells/mL (c) 900 cells/mL (d) 1200 cells/mL 48. What is the purpose of serial dilutions prior to measuring bacterial growth? (a) to make the culture less turbid and allow spectrophotometric measurements (b) to allow filtration of the culture and subsequent plating of cells (c) to dilute the culture to allow distinguishable colonies for counting (d) all of the above 49. How would a Petroff-Hausser counting chamber (hemocytometer) allow you to measure bacterial growth? (a) allows you to count CFUs after appropriate dilution (b) allows you to count cells directly after appropriate dilution (c) allows you to estimate growth based on how turbid the growth medium is (d) allows you to estimate growth based on dry weight 50. Which of the following techniques does not differentiate between viable (living) and dead bacterial cells? (a) spread-plate (b) streak-plate (c) pour-plate (d) turbidity 51. The streak-plate method produces pure cultures by separating individual bacterial cells from one another by… (a) dispersing them in liquid media (b) killing off those cells that are not able to survive in hot agar (c) using a spectrophotometer (d) dragging them across a solid surface 52. Which of the following types of bacteria would you expect to optimally grow at temperatures below 20*C (cold)? (a) psychrophile (b) mesophile (c) thermophile (d) extreme thermophile 53. A microbe that grows best when there is a small amount of oxygen available is known as… (a) an obligate aerobe (b) a microaerophile (c) a facultative anaerobe (d) an aerotolerant 54. Which of the following can survive in the deep layers of mud, where there is a complete lack of free oxygen? (a) aerobe facultative anaerobe (c) obligate anaerobe (d) b&c 55. A barophile is an organism that… (a) can grow under high hydrostatic pressure (b) can grow in high salt concentrations (c) can grow in dry conditions (d) can grow under radiation 56. Organisms with special nutritional needs and are difficult to grow in the laboratory are said to be… (a) fastidious (b) halophiles (c) barophiles (d) aerotolerant 57. Excenyzmes are typically hydrolases because… (a) bacteria cannot use large molecules (b) it allows bacteria to breakdown large molecules and then use it (c) exoenzymes are used inside the cell to digest food (d) a & b 58. A decrease in the amount of nitrogen to a culture of Clostridium botulinum would induce… (a) germination (b) activation (c) outgrowth (d) sporulation 59. The EMB agar partially inhibits gram-positive bacteria and allows us to distinguish between lactose-fermenters and nonfermenters. This medium is classified as… (a) selective only (b) differential only c) selective & differential (d) none of the above
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