A 46-year-old wom an w ho had undergone a radical m astectomy for in ltrating ductal carcinom a started adjuvant com bination therapy w ith the CMF (cyclophospham ide, m ethotrexate, and uorouracil) regim en. Which of the follow ing was the initial m olecular action that m ost likely m ediated the therapeutic e ect of m ethotrexate in the patients disease? A. Inhibition of purine biosynthesis B. Inhibition of DNA chain elongation C. Inhibition of single-stranded DNA resealing D. DNA fragm entation through free radical form ation E. Inhibition of m icrotubule assem bly
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